Lucie Kuchařová studied cello at the Janáček Conservatory, the Academy of Music and Performing Arts, Bratislava, and the Masaryk University, Brno, followed by the University of Copenhagen, Conservatorio Giuseppe Tartini and Landestheater Detmold.

She became a member of the Philharmonie Junge Donau, Radio Symphony Orchestra Bratislava, National Opera Theatre Bratislava, Prague Royal Philharmonic, Opera Giocosa del Friuli Venezia Giulia and Orchestra Sinfonica Giuseppe Verdi, Milan. Later Lucie was engaged by the Orquestra do Norte, Portugal.

In 2009 Lucie joined the Malta Philharmonic Orchestra. Concerts of note were at the Warsaw Autumn Festival, Placido Domingo in Lisbon, the Vatican, Tonhalle Zürich, Osaka Festival Hall, Teatro la Fenice, Musikverein, Berlin Philharmonic Hall, Moscow State Conservatory, Maarinski Theatre and Carnegie Hall.